Pee-wee's Big Adventure Biker #2: I say we kill him!
Biker Gang: YEAH!
Biker #3: I say we hang him, then we kill him!
Biker Gang: YEAH!
Biker #4: I say we stomp him!
Biker Gang: YEAH!
Biker #4: Then we tattoo him!
Biker Gang: YEAH!
Biker #4: Then we hang him!
Biker Gang: YEAH!!
Biker #4: And then we kill him!
Biker Gang: YEAH!!!
Pee-wee: [tries to throw his voice without moving his lips] I say we let him go.
Biker Gang: NO!!!
Biker Mama: [whistles and grabs Pee-wee] I say ya let ME have him first!
(the Biker Gang breaks out in raucous laughter)
Pee-wee: Wait! Don't I get a last request?
Lead Biker: [turns to his gang, then to Pee-wee] Why not?

Anchorman The Legend of Ron Burgundy Angry Biker: What do you love?
Ron Burgundy: I love poetry, and a glass of scotch, and, of course, my friend Baxter here.
Angry Biker: Well, now, guess what, this is happening.
[grabs Baxter]
Ron Burgundy: Excuse me, excuse me, what are you doing?
[biker punts Baxter over bridge]
Angry Biker: That's how I roll!

Mystery Science Theater 3000 [On the beach, the biker-gang leader fights lean Hank]
Servo [as Biker/Johnny Mathis]: [singing to "Chances Are"]

Chances are
That I'll kick your scrawny ass...

Pee-wee's Big Adventure [Pee-wee enters a biker bar and tries to use the payphone but can't because of the noise]
Pee-wee: SHH!!!!
[the bikers all stop and stare at Pee-wee]
Pee-wee: I'm trying to use the phone!!
[the bikers walk up to him; one of the bikers hangs up the phone]
Biker 1: Did anybody tell you that this is the private club of The Satan's Helpers?
Pee-wee: Nobody hipped me to that, dude.
Biker 1: It's off-limits!

Angel Wesley: Feel natural?
Faith: Eh, it's like riding a biker.

Mystery Science Theater 3000 Biker: [to Ross Hagen] Where ya from?
Servo: Sidehackers.

Fletch Lives aka Fletch 2 (Fletch, dressed as a nerdy businessman, has walked into a biker bar)

Wedding Singer, The [Billy Idol is blocking Glenn from interfering in Robbie's declaration of love to Julia with a airplane food trolley]
Billy Idol: How you doin', sir - chicken or fish?
Glenn: You better get out of my way, Billy. You're gonna get hurt!
Billy Idol: Oh yeah?
[A huge biker wearing a Billy Idol T-shirt gets out of his seat and interjects himself between Billy and Glenn]
Biker: Don't you talk to Billy Idol that way!

Buffy the Vampire Slayer film "Hey, babe, you wanna get some real power between your legs?" ~ Biker

Super Troopers oh,Biker... I'm an idiot. [when Foster realizes that he misunderstood Ursula's disguise suggestion]